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Inserts encoding the neoAgs were obtained from Genscript and cloned into MSCV-IRES-Thy1.1 DEST and pBK-T-SFV-b12A using BglII&SalI and Apa I websites, respectively. MSCV-IRES-Thy1.1 DEST was a present from Anjana Rao (Addgene plasmid # 17442). Plasmids pMSCV-TMGP69-IRES-Thy1.1, pMSCV-TMGP73-IRES-Thy1.1 encoding the neoAgs and control plasmid (MSCV-IRES-Thy1.1 DEST) have been used to prepare retrovirus for transduction experiments. As a library, NLM offers access to scientific literature. Inclusion in an NLM database doesn’t suggest endorsement of, or agreement with, the contents by NLM or the National Institutes of Health. Federal authorities web sites typically finish in .gov or .mil.
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It has been proposed that neoAg-specific T cells play a prominent role in ICI efficacy, since TMB correlates with the response rate to these therapies . Analyses of expression of mutated sequences revealed that about 65% of mutations have been situated at expressed genes. A high variability relating to expression of mutated genes with potential neoAg-coding capability has been reported in TNBC, ranging from 35-50% in some circumstances , to greater than 80% in others .
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